Hybrid Optically-Gated Light Sheet Microscopy Allows Long-term Timelapse Imaging in the Developing Zebrafish Heart

Abstract

Although the low-impact nature of light sheet microscopy has opened up new avenues for developmental timelapse imaging, the heart remains a particularly challenging organ to image in 3D timelapse. To image processes on timescales of minutes to hours (such as heart development, cell migration, repair and regeneration) demands some form of synchronized image acquisition in order to separate the high-frequency heartbeat motion from the lower-speed morphological changes of interest. Although current postacquisition synchronization methods are attractive for imaging the beat process, or for acquiring small numbers of timepoints, the accumulated light dose precludes longer-term timelapse imaging. Indeed, we will show that this rapidly induces catastrophic photobleaching, phototoxicity and heart arrhythmia.

Date
2018-01-01
Event
10th Anniversary Light Sheet Fluorescence Microscopy Conference
Location
Frankfurt am Main, Germany
Chas Nelson
Chas Nelson
LKAS Research Fellow in Data Science

Interdisciplinary scientist with a background in bioimaging and informatics.

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