oral

Hybrid Optical Gating Allows 4D Imaging and Quantification in the Developing Zebrafish Heart

Researchers interested in the heart have long used a wide range of imaging techniques to see, understand and quantify changes throughout heart development, repair and, in certain species, regeneration. Two major challenges in imaging the heart are …

Imaging the developing heart: synchronized time-lapse microscopy during developmental changes

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture …

Hybrid Optically-Gated Light Sheet Microscopy Allows Long-term Timelapse Imaging in the Developing Zebrafish Heart

Although the low-impact nature of light sheet microscopy has opened up new avenues for developmental timelapse imaging, the heart remains a particularly challenging organ to image in 3D timelapse. To image processes on timescales of minutes to hours …

Application of High-Speed Level Set Segmentation to Light Sheet Fluorescence Microscopy

Light sheet fluorescence microscopy produces large, detailed 3D images of biological samples, but these images are often heavily afflicted by structured noise caused by optical effects such as attenuation, refraction and shadowing. Local Gaussian …

A Bioimage Informatics QVEST: Quick, Versatile and Easy Segmentation & Tracking System

Often bioimage informatics solutions are developed on a case-by-case system and, once complete, little research goes in to developing the systems for a wide spread of applications. Here we demonstrate how a single image analysis technique can be used …