Adaptive Prospective Optical Gating

Adaptive prospective optical gating ensures phase-locked imaging over day-long developmental periods.

As Research Assistant in Microscopy and Optics to Dr Jonathan Taylor (Glasgow University) I worked on real-time image processing for the synchronisation of 3D images of the in vivo, beating zebrafish heart. Through this project we have developed a technique we call adaptive prospective optical gating.

The constant motion of the heart is a major obstacle to live imaging and, in the past, fish have been fixed or their heartbeats have been pharmaceutically slowed down. Our research aims to develop the hardware and software needed to create truly synchronised 3D images of whole heartbeats over long time periods so that the long term effects of drugs or injury on heart and vasculature can be elucidated.

I am now investing a large amount of time and effort in making this system fully open source and sustainable for future developers and users.

This research was funded by the British Heart Foundation and my open source efforts by my LKAS Research Fellowship in Data Science (University of Glasgow).

Chas Nelson
Chas Nelson
LKAS Research Fellow in Data Science

Interdisciplinary scientist with a background in bioimaging and informatics.