Adaptive prospective optical gating ensures phase-locked imaging over day-long developmental periods.

Adaptive Prospective Optical Gating

Adaptive prospective optical gating ensures phase-locked imaging over day-long developmental periods.

Adaptive Prospective Optical Gating

As Research Assistant to Dr Jonathan Taylor (Glasgow University) I worked on real-time image processing for the synchronisation of 3D images of the in vivo, beating zebrafish heart. Through this project we have developed a technique we call adaptive prospective optical gating.

The constant motion of the heart is a major obstacle to live imaging and, in the past, fish have been fixed or their heartbeats have been pharmaceutically slowed down. Our research aims to develop the hardware and software needed to create truly synchronised 3D images of whole heartbeats over long time periods so that the long term effects of drugs or injury on heart and vasculature can be elucidated.

This research was funded by the British Heart Foundation.

A preprint of this work can be found on bioRxiv.

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Chas Nelson
LKAS Research Fellow in Data Science

An interdisciplinary scientist with a background in quantitative microscopy and bioimage analysis.

Publications

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, …

Talks

Although fluorescence microscopy has opened up new avenues for developmental imaging, the heart remains a particularly challenging …

Two major challenges in imaging the living, beating heart are the contrasting problems of high-frequency heart beating and …

It has long been argued by poets, ancient Egyptians and cardiologists that the heart is the most important organ. They’re …

Researchers interested in the heart have long used a wide range of imaging techniques to see, understand and quantify changes …

Biology is the study of life and most life is four-dimensional (space and time), but for years it has been studied using dead samples …

Researchers interested in the heart have long used a wide range of imaging techniques to see, understand and quantify changes …

Microscopy and imaging provide some of the most visually exciting and scientifically informative data available to bioscientists. Many …

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, …